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The series focuses on Dr. Jason Cole (Steven Pasquale), a successful neurosurgeon with a secret. Every night at 8:25 p.m., and lasting exactly 12 hours, Cole is switched into an alternate, evil personality named Ian Price. Cole has been able to suppress Price by injecting a strong pharmaceutical mixture that sedates their shared body, rendering it impossible for the evil alter-ego to function. But one night Cole discovers that their body has grown immune to the drug and Price has emerged in a rage. Angry at having been suppressed, Cole's alternate personality is focused on wreaking havoc on Cole's life, creating problems which could cost him his romance, career, and even his life.

The series is a modern take on the classic Strange Case of Dr Jekyll and Mr Hyde story.